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ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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MedChemExpress dichlorodihydro fluorescein diacetate dcfh da reactive oxygen species
ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. (D–E) DCFH-DA fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Ultra-small iron-based nanoparticles Mitigate rheumatoid arthritis inflammation via macrophage Repolarization and SLC7A11/GPX4-mediated ferroptosis inhibition

doi: 10.1016/j.mtbio.2025.102551

Figure Lengend Snippet: ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. (D–E) DCFH-DA fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The ability of Fe 3 O 4 @C to scavenge ROS was assessed utilizing the DCFH-DA fluorescent probe (MedChemExpress, USA).

Techniques: CCK-8 Assay, Staining, Fluorescence, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay